PSA ELISA Kit (55R-BC2601)

ELISA kit for detection of PSA in the research laboratory

Synonyms Prostate Specific Antigen ELISA Kit, Total PSA ELISA Kit
Applications ELISA
Assay Information The PSA ELISA test is a solid phase two-site immunoassay. An anti-PSA monoclonal antibody is coated on the surface of the microtiter wells and a mouse anti-PSA antibody labeled with horseradish peroxidase is used as the tracer. The PSA molecules present in the standard solution or sera are 'sandwiched' between the two antibodies. Following the formation of the coated antibody-antigen-antibody-enzyme complex, the unbound proteins and antibody-enzyme tracers are removed by washing. The horseradish peroxidase activity bound in the wells is then assayed by a colorimetric reaction. The intensity of the color formed is proportional to the concentration of PSA present in the sample.

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Store at 4 deg C.
Biohazard Information All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potentia

General Information

Biological Significance PSA is a 32 kDa single chain glycoprotein serine protease with a chymotrypsin like specificity produced by the secretory epithelium of the prostate gland. Only low levels of PSA are normally present in the blood stream, and increasing serum concentrations indicate prostatic pathology, including benign prostatic hyperplasia and cancer of the prostate.

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PSA ELISA Kit Protocol

Added: Sep 29, 2015

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Availability: In stock

Price: £156.24
Size: 96 wells
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