RAB3D protein (His tag) (80R-1861)
Purified recombinant RAB3D protein
|Synonyms||RABD-3 protein, Ras-related protein Rab-3D protein, RAB16 protein, RAB3D, RABD 3 protein, GOV protein, RABD-3, RABD 3|
Coomassie Blue stained SDS-PAGE of RAB3D protein (His tag) (80R-1861)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-219 amino acids: MGSSHHHHHH SSGLVPRGSH MASAGDTQAG PRDAADQNFD YMFKLLLIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTVYRHDKR IKLQIWDTAG QERYRTITTA YYRGAMGFLL MYDIANQESF AAVQDWATQI KTYSWDNAQV ILVGNKCDLE DERVVPAEDG RRLADDLGFE FFEASAKENI NVKQVFERLV DVICEKMNES LEPSSSSGSN GKGPAVGDAP APQPSSCSC|
|Grade & Purity||> 90% pure|
|Molecular Weight||26.4 kDa (239aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 30% glycerol and 0.2M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ras-related protein Rab-3D, also known as GOV, is a member of the Ras superfamily of small Rab GTPases. Rab proteins play an important role either in endocytosis or in biosynthetic protein transport. Rab3D mRNA is predominantly expressed in white adipose tissue and, coincident with GLUT4, increases upon differentiation of 3T3-L1 broblasts into insulin-responsive adipocytes. Rab3D displays close structural similarities with Rab 3A, an isoform which has been functionally implicated in regulated exocytosis in various cell types. Recombinant human Rab3D protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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