Rabbit anti Human IgG (H + L) (biotin) (43C-CB0912)

Rabbit anti Human IgG (H + L) secondary antibody (biotin)

Synonyms Rabbit anti Human Immunoglobulin G (H + L) secondary antibody (biotin)
Specificity Human IgG (H + L)
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen Rabbit anti-human IgG (H+L) (biotin) was raised in rabbit using human IgG whole molecule as the immunogen.

Images

Western blot analysis using Rabbit anti Human IgG (H + L) (biotin) (43C-CB0912)

Western Blot of Biotin Conjugated Human IgG antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Human IgG antibody biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Human IgG..

Specifications

Host Rabbit
Method of Purification Rabbit anti Human IgG (H + L) (biotin) was purified by affinity chromatography.
Tag/Conjugate Biotin
Form & Buffer Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl, 10 mg/ml BSA, and 0.01% thimerisol. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains ? and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western blot analysis using Rabbit anti Human IgG (H + L) (biotin) (43C-CB0912) | Western Blot of Biotin Conjugated Human IgG antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Human IgG antibody biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Human IgG..

Availability: In stock

Price: £188.29
Size: 2 mg
OR
Shipping
View Our Distributors