RALA protein (His tag) (80R-1583)
Purified recombinant Human RALA protein
|Synonyms||Ras-related protein Ral-A protein, RAL|
Coomassie Blue stained SDS-PAGE of RALA protein (His tag) (80R-1583)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-203 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMAANKP KGQNSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLCVFSIT EMESFAATAD FREQILRVKE DENVPFLLVG NKSDLEDKRQ VSVEEAKNRA EQWNVNYVET SAKTRANVDK VFFDLMREIR ARKMEDSKEK NGKKKRKSLA KRIRERC|
|Grade & Purity||> 90% pure|
|Molecular Weight||31.8 kDa (291aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT, 100mM NaCl, and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RALA (Ras-related protein Ral-A) belongs to the small GTPase superfamily, Ras family of proteins. RALA mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. RALA is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis. Recombinant human RALA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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