RALB protein (His tag) (80R-1599)
Purified recombinant Human RALB protein
|Synonyms||Ras-related protein Ral-B protein,|
Coomassie Blue stained SDS-PAGE of RALB protein (His tag) (80R-1599)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-203 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMAANKS KGQSSLALHK VIMVGSGGVG KSALTLQFMY DEFVEDYEPT KADSYRKKVV LDGEEVQIDI LDTAGQEDYA AIRDNYFRSG EGFLLVFSIT EHESFTATAE FREQILRVKA EEDKIPLLVV GNKSDLEERR QVPVEEARSK AEEWGVQYVE TSAKTRANVD KVFFDLMREI RTKKMSENKD KNGKKSSKNK KSFKERC|
|Grade & Purity||> 90% pure|
|Molecular Weight||25.6 kDa (227aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RALB, also called Ras-related protein Ral-B, belongs to subfamily of Ras-related GTPases. This protein is activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. It plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Also it is required for suppression of apoptosis. Recombinant human RALB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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