RASSF1A antibody (10R-1008)

Mouse monoclonal RASSF1A antibody

Synonyms Monoclonal RASSF1A antibody, Anti-RASSF1A antibody, REH3P21 antibody, Ras association (RalGDS/AF-6) domain family member 1 antibody, RASSF1(Ras association (RalGDS/AF-6) domain family 1) antibody, RASSFA-1 antibody, RDA32 antibody, 123F2 antibody, RASSF1A, RASSFA 1 antibody, RASSFA-1, RASSF1A antibody, NORE2A antibody, RASSFA 1
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen RASSF1A antibody was raised in mouse using recombinant human RASSF1A (1-340aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of RASSF1A antibody (10R-1008)

Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone 3F3
Isotype IgG1 kappa
Source Anti-human RASSF1A antibody, clone 3F3, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant RASSF1A.
Method of Purification RASSF1A antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.

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Images

  • Western Blot analysis of RASSF1A antibody (10R-1008) | Cell lysates of HEk293(1), A549(2) and HeLa(3) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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