RCN2 protein (His tag) (80R-1754)
Purified recombinant Human RCN2 protein
|Synonyms||RCN-2 protein, RCN 2, RCN2, RCN-2, E6BP protein, ERC55 protein, RCN 2 protein, Reticulocalbin-2 protein|
Coomassie Blue stained SDS-PAGE of RCN2 protein (His tag) (80R-1754)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||26-317 amino acids: MGSSHHHHHH SSGLVPRGSH MEELHYPLGE RRSDYDREAL LGVQEDVDEY VKLGHEEQQK RLQAIIKKID LDSDGFLTES ELSSWIQMSF KHYAMQEAKQ QFVEYDKNSD DTVTWDEYNI QMYDRVIDFD ENTALDDAEE ESFRKLHLKD KKRFEKANQD SGPGLSLEEF IAFEHPEEVD YMTEFVIQEA LEEHDKNGDG FVSLEEFLGD YRWDPTANED PEWILVEKDR FVNDYDKDND GRLDPQELLP WVVPNNQGIA QEEALHLIDE MDLNGDKKLS EEEILENPDL FLTSEATDYG RQLHDDYFYH DEL|
|Grade & Purity||> 90% pure|
|Molecular Weight||36.8 kDa (313aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||RCN2, also known as reticulocalbin-2, belongs to the CREC protein family. RCN2 is a calcium-binding protein located in the lumen of the ER. This protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. Recombinant human RCN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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