RhoD protein (His tag) (80R-1760)
Purified recombinant Human RhoD protein
|Synonyms||RHOHP1 protein, RHOM protein, ARHD protein, Rho-related GTP-binding protein RhoD protein|
Coomassie Blue stained SDS-PAGE of RhoD protein (His tag) (80R-1760)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||18-207 amino acids: MGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH DNVHAVFQEA AEVALSSRGR NFWRRITQGF C|
|Grade & Purity||> 90% pure|
|Molecular Weight||23.8 kDa (211aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 5mM DTT, 50% glycerol, 200mM NaCl, 2mM EDTA and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Rho-related GTP-binding protein, also known as RhoD, belongs to the small GTPase superfamily. The small GTPase Rho D contributes to rearrangement of the actin cytoskeleton and cell surface and also governs endosome motility and distribution. Recombinant human RhoD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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