rnhA protein (His tag) (80R-1934)

Purified recombinant rnhA protein

Synonyms Ribonuclease HI protein, sin protein, rnh protein, dasF protein, degrades RNA of DNA-RNA hybrids. protein, herA protein, JW0204 protein, cer protein, sdrA protein, ECK0214 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE
Assay Information rnhA protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Images

Coomassie Blue stained SDS-PAGE of rnhA protein (His tag) (80R-1934)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-155 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 20 kDa
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 10% glycerol and 2mM DTT.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant E. coli rnhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of rnhA protein (His tag) (80R-1934) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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