RPP30 protein (His tag) (80R-1582)

Purified recombinant Human RPP30 protein

Synonyms RPP30, RPP 30 protein, RPP-30, RPP-30 protein, TSG15, RNASEP2 protein, RPP 30, Ribonuclease P protein subunit p30 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of RPP30 protein (His tag) (80R-1582)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-268 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMAVFADL DLRAGSDLKA LRGLVETAAH LGYSVVAINH IVDFKEKKQE IEKPVAVSEL FTTLPIVQGK SRPIKILTRL TIIVSDPSHC NVLRATSSRA RLYDVVAVFP KTEKLFHIAC THLDVDLVCI TVTEKLPFYF KRPPINVAID RGLAFELVYS PAIKDSTMRR YTISSALNLM QICKGKNVII SSAAERPLEI RGPYDVANLG LLFGLSESDA KAAVSTNCRA ALLHGETRKT AFGIISTVKK PRPSEGDEDC LPASKKAKCE G
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 31.8 kDa (291aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 5mM DTT, 200mM NaCl and 1mM EDTA.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance RPP30 (Ribonuclease P protein subunit p30) belongs to the eukaryotic/archaeal RNase P protein component 3 family. RPP30 is component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Ribonuclease P (PNase P) is small nuclear ribonucleoproteins (snRNPs) that act on RNA substrates in vitro. Also, RNase P accumulates in the nucleolus, have a similar RNA component and also have several protein subunits in common. Recombinant human RPP30 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of RPP30 protein (His tag) (80R-1582) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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