SFPQ antibody (70R-1373)

Rabbit polyclonal SFPQ antibody

Synonyms Polyclonal SFPQ antibody, Anti-SFPQ antibody, PSF antibody, Polypyrimidine Tract Binding Protein Associated antibody, Splicing Factor Proline/Glutamine-Rich antibody, POMP100 antibody
Cross Reactivity Human,Mouse,Rat
Applications IHC, WB
Immunogen SFPQ antibody was raised using a synthetic peptide corresponding to a region with amino acids PVIVEPLEQLDDEDGLPEKLAQKNPMYQKERETPPRFAQHGTFEYEYSQR
Assay Information SFPQ Blocking Peptide, catalog no. 33R-7413, is also available for use as a blocking control in assays to test for specificity of this SFPQ antibody

Images

Immunohistochemical staining using SFPQ antibody (70R-1373)

SFPQ antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Skeletal muscle cells (arrows) in Human Muscle. Magnification is at 400X

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 78 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of SFPQ antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 5 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.

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Images

  • Immunohistochemical staining using SFPQ antibody (70R-1373) | SFPQ antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Skeletal muscle cells (arrows) in Human Muscle. Magnification is at 400X
  • Western Blot analysis using SFPQ antibody (70R-1373) | SFPQ antibody (70R-1373) used at 5 ug/ml to detect target protein.

Availability: In stock

Price: £220.34
Size: 100 ug
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