SHH protein (His tag) (80R-1302)

Purified recombinant Human SHH protein

Synonyms HPE 3 protein, sonic hedgehog homolog (Drosophila) protein, TPT protein, Holoprosencephaly 3 protein, MCOPCB5 protein, SHH protein, Hhg1 protein, HLP3 protein, SMMCI protein, HLP 3 protein, HPE3 protein, Sonic Hedgehog (Drosophila) homolog protein, TPTPS., Sonic hedgehog homolog protein, Dsh protein, Hx13 protein, Hx protein, SMMC I protein, HHG1 protein, Sonic hedgehog protein HHG 1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Specifications

Residues 25-198 amino acids: MCGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKITRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGGLEHHH HHH
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 20.8 kDa (183aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SHH is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH). This protein is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis. SHH contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Recombinant mouse SHH protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Availability: In stock

Price: £176.28
Size: 100 ug
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