SlyD protein (30R-1170)

Purified recombinant E.coli SlyD protein

Synonyms WHP., FKBP-type peptidyl-prolyl cis-trans isomerase FKBP type peptidyl prolyl cis trans isomerase slyD protein, Histidine rich protein protein, FKBP-type peptidyl-prolyl cis-trans isomerase protein, Rotamase protein, PPIase protein, SlyD protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant SlyD protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes

Images

Coomassie Blue stained SDS-PAGE of SlyD protein (30R-1170)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-196 amino acids: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 21 kDa (196 aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris, pH 7.5.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 66 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. coli slyD was expressed in E. coli and purified by conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of SlyD protein (30R-1170) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £236.37
Size: 100 ug
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