SNRPE protein (His tag) (80R-1677)
Purified recombinant Human SNRPE protein
|Synonyms||B-raf protein, Sm-E protein, Small nuclear ribonucleoprotein E protein, SME|
Coomassie Blue stained SDS-PAGE of SNRPE protein (His tag) (80R-1677)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-92 amino acids: MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN|
|Grade & Purity||> 95% pure|
|Molecular Weight||12.9 kDa (112aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT, 1mM EDTA and 30% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||SNRPE, also known as SME, aids in the cytoplasmic construction of the UsnRNPs by binding to a conserved Sm site on UsnRNA and forming a stable snRNP core complex. As a core protein to UsnRNP, the SNRPE associates with the entire U family of snRNAs including U1–U6. SNRPE also interacts with DDX20 and Small nuclear ribonucleoprotein polypeptide F. Recombinant human SNRPE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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