SOD2 protein (His tag) (80R-1279)

Purified recombinant Human SOD2 protein

Synonyms SOD 2, SOD2, SOD-2, SOD 2 protein, SOD-2 protein, Superoxide dismutase 2 mitochondrial protein, IPOB protein, SOD 2 protein, MNSOD protein, Superoxide dismutase [Mn] mitochondrial protein, SOD2 protein, Superoxide Dismutase-2, Manganese superoxide dismutase protein, MVCD6 protein, Mn SOD protein, mitochondrial IPO B protein, Superoxide dismutase [Mn] protein, Manganese SOD protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochromC, 0.05mM xanthine, 0.005 units xanthine oxidase. 2. Equilibrate to 25 DEG C and monitor at A550nm until the value is constant using a spectrophotometer. 3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

Images

Coomassie Blue stained SDS-PAGE of SOD2 protein (His tag) (80R-1279)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 25-222 amino acids: MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 24.4 kDa (219aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 1,200 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25 DEG C in a 1.5 ml reaction volume.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human SOD2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of SOD2 protein (His tag) (80R-1279) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £176.28
Size: 100 ug
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