SRI protein (His tag) (80R-1699)
Purified recombinant Human SRI protein
|Synonyms||V19 protein, SCN protein, CP22 protein, 22 kDa protein, Sorcin protein|
Coomassie Blue stained SDS-PAGE of SRI protein (His tag) (80R-1699)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-198 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMAYPGHP GAGGGYYPGG YGGAPGGPAF PGQTQDPLYG YFAAVAGQDG QIDADELQRC LTQSGIAGGY KPFNLETCRL MVSMLDRDMS GTMGFNEFKE LWAVLNGWRQ HFISFDTDRS GTVDPQELQK ALTTMGFRLS PQAVNSIAKR YSTNGKITFD DYIACCVKLR ALTDSFRRRD TAQQGVVNFP YDDFIQCVMS V|
|Grade & Purity||> 90% pure|
|Molecular Weight||24.1 kDa (221aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 40% glycerol, 0.2M NaCl, 1mM DTT and 0.1mM PMSF.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method )|
|Biological Significance||Sorcin, also known as SRI, is a 22-kDa calcium-binding protein initially identified in multidrug-resistant cells. SRI modulates excitation-contraction coupling in the heart and contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Recombinant human SRI protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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