Streptavidin protein (Cy5.5) (65C-CE0307)

Purified homogeneous preparation of Cy5.5 conjugated Streptavidin protein

Species Streptomyces avidinii
Protein Type Binding Protein
Applications ELISA, FC, IF

Specifications

Method of Purification Streptavidin protein (Cy5.5) was purified by Protein A affinity chromatography.
Tag/Conjugate CY5.5
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7); the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of ˜10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.

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Availability: In stock

Price: £236.37
Size: 1 mg
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