TALDO1 protein (His tag) (80R-1428)
Purified recombinant Human TALDO1 protein
|Synonyms||TALDO 1 protein, TALDO-1 protein, TALDOR protein, TALDO1, TAL protein, TAL-H protein, TALH protein, TALDO 1, Transaldolase 1, TALDO1 protein, TALDO-1|
Coomassie Blue stained SDS-PAGE of TALDO1 protein (His tag) (80R-1428)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-337 amino acids: MGSSHHHHHH SSGLVPRGSH MSSSPVKRQR MESALDQLKQ FTTVVADTGD FHAIDEYKPQ DATTNPSLIL AAAQMPAYQE LVEEAIAYGR KLGGSQEDQI KNAIDKLFVL FGAEILKKIP GRVSTEVDAR LSFDKDAMVA RARRLIELYK EAGISKDRIL IKLSSTWEGI QAGKELEEQH GIHCNMTLLF SFAQAVACAE AGVTLISPFV GRILDWHVAN TDKKSYEPLE DPGVKSVTKI YNYYKKFSYK TIVMGASFRN TGEIKALAGC DFLTISPKLL GELLQDNAKL VPVLSAKAAQ ASDLEKIHLD EKSFRWLHNE DQMAVEKLSD GIRKFAADAV KLERMLTERM FNAENGK|
|Grade & Purity||> 90% pure|
|Molecular Weight||39.7 kDa(357aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||TALDO1 is a key enzyme of the non-oxidative pentose phosphate pathway providing ribose-5-phosphate for nucleic acid synthesis and NADPH for lipid biosynthesis. This protein transfers a dihydroxyacetone group from donor compounds (fructose 6-phosphate or sedoheptulose 7-phosphate) to aldehyde acceptor compounds. It is expressed at selectively high levels in oligodendrocytes of the brain. Deficiency of TALDO1 causes accumulation of erythritol, D-arabitol, and ribitol. Recombinant human TALDO1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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