TIGAR protein (His tag) (80R-1596)
Purified recombinant Human TIGAR protein
|Synonyms||TP53-induced glycolysis and apoptosis regulator protein,|
Coomassie Blue stained SDS-PAGE of TIGAR protein (His tag) (80R-1596)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-270 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMARFAL TVVRHGETRF NKEKIIQGQG VDEPLSETGF KQAAAAGIFL NNVKFTHAFS SDLMRTKQTM HGILERSKFC KDMTVKYDSR LRERKYGVVE GKALSELRAM AKAAREECPV FTPPGGETLD QVKMRGIDFF EFLCQLILKE ADQKEQFSQG SPSNCLETSL AEIFPLGKNH SSKVNSDSGI PGLAASVLVV SHGAYMRSLF DYFLTDLKCS LPATLSRSEL MSVTPNTGMS LFIINFEEGR EVKPTVQCIC MNLQDHLNGL TETR|
|Grade & Purity||> 90% pure|
|Molecular Weight||32.6 kDa (294aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 2mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||TIGAR, also known as TP53-induced glycolysis and apoptosis regulator, is a 270 amino acid protein induced by the p53 tumor suppressor pathway that functions to protect against oxidative stress. TIGAR specifically functions to block glycolysis, leading the pathway to the pentose phosphate shunt and decreasing the intracellular concentration of reactive oxygen species. These facts indicate that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. Recombinant human TIGAR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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