TNF alpha protein (30R-AT045)

Purified recombinant Human TNF alpha protein

Synonyms Tumor Necrosis Factor protein, TNFa protein, Cytotoxin protein, DIF protein, TNFSF2 protein, TNF-alpha protein, TNF-a protein, Differentiation-inducing factor protein, Necrosin protein, Cachectin protein
Species Human
Protein Type Recombinant
Applications User optimized
Assay Information Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.

Specifications

Residues Tumor Necrosis Factor-a Human Recombinant protein containing 158 amino acids.
Expression System E.coli
Grade & Purity > 98% pure
Method of Purification TNF alpha protein was purified by a proprietary chromatographic technique.
Molecular Weight 17,483 Da
Form & Buffer Lyophilized from 20mM PBS, pH 7.2, with 10mM NaCl. Reconstitute in water to a concentration of 0.1 - 1.0mg/ml.

Usage & Assay Information

Bioactivity The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

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Availability: In stock

Price: £1,277.99
Size: 1 mg
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