TPX protein (His tag) (80R-1814)
Purified recombinant E.coli TPX protein
|Synonyms||JW1317 protein, yzzJ protein, Lipid hydroperoxide peroxidase. protein, ECK1320 protein|
Coomassie Blue stained SDS-PAGE of TPX protein (His tag) (80R-1814)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-168 amino acids: MGSSHHHHHH SSGLVPRGSH MSQTVHFQGN PVTVANSIPQ AGSKAQTFTL VAKDLSDVTL GQFAGKRKVL NIFPSIDTGV CAASVRKFNQ LATEIDNTVV LCISADLPFA QSRFCGAEGL NNVITLSTFR NAEFLQAYGV AIADGPLKGL AARAVVVIDE NDNVIFSQLV DEITTEPDYE AALAVLKA|
|Grade & Purity||> 95% pure|
|Molecular Weight||19.9 kDa (188aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||TPX, also known as lipid hydroperoxide peroxidase, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an important role in thioredoxin peroxidase activity. Recombinant E. coli TPX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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