TREX2 protein (His tag) (80R-1728)
Purified recombinant Human TREX2 protein
|Synonyms||TREX 2, TREX 2 protein, TREX2, Three prime repair exonuclease 2 protein, TREX-2, TREX-2 protein|
Coomassie Blue stained SDS-PAGE of TREX2 protein (His tag) (80R-1728)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-236 amino acids: MGSSHHHHHH SSGLVPRGSH MSEAPRAETF VFLDLEATGL PSVEPEIAEL SLFAVHRSSL ENPEHDESGA LVLPRVLDKL TLCMCPERPF TAKASEITGL SSEGLARCRK AGFDGAVVRT LQAFLSRQAG PICLVAHNGF DYDFPLLCAE LRRLGARLPR DTVCLDTLPA LRGLDRAHSH GTRARGRQGY SLGSLFHRYF RAEPSAAHSA EGDVHTLLLI FLHRAAELLA WADEQARGWA HIEPMYLPPD DPSLEA|
|Grade & Purity||> 95% pure|
|Molecular Weight||28.0 kDa (256aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 5mM DTT and 30% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||TREX2 is an enzyme containing 3-prime-to-5-prime exonuclease activity and removes mismatched, modified, fragmented, and normal nucleotides to generate the appropriate 3-prime termini for subsequent steps in the DNA metabolic pathways. This enzyme is involved in DNA replication, repair, and recombination. Recombinant human TREX2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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