TYMS antibody (10R-1166)

Mouse monoclonal TYMS antibody

Synonyms Monoclonal TYMS antibody, Anti-TYMS antibody, Tsase antibody, TMS antibody, Anti TYMS antibody , TYMS protein antibody, TYMS antibody, MGC88736 antibody, TYMS protein antibody, Tyms thymidylate synthetase antibody, d TMP synthase antibody, EC 2.1.1.45 antibody, HST422 antibody, Thymidylate synthetase antibody, HST422 antibody, TS antibody, MGC88736 antibody, Thymidylate synthase antibody, TYMS antibody, TYMS antibody, EC 2.1.1.45 antibody, Thymidylate synthase antibody, TS antibody, Tyms thymidylate synthetase antibody, TMS antibody, Thymidylate synthetase antibody, Tsase antibody, d TMP synthase antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen TYMS antibody was raised in mouse using recombinant TYMS (1-313aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of TYMS antibody (10R-1166)

Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human TYMS (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT21S5
Isotype IgG2b kappa
Source Anti-human TYMS antibody, clone AT1S5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TYMS protein.
Method of Purification TYMS antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:500-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dMTP) is an essential step for the formation of thymine nucleotides. This process is catalyzed by thymidylate synthase (TS or TYMS). TYMS is an intracellular enzyme that provides the sole de novo source of thymidylate in proliferating cells. Being the exclusive source of dTMP, TS is also an important target for anticancer agents such as 5-fluorouracil (5-FU). 5-FU acts as a TS inhibitor and is active against solid tumors such as colon, breast, head and neck. TS expression may be useful in predicting overall patient survival, but the prognostic value of TS expression continues to be a subject of investigation for different types of cancers.

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Images

  • Western Blot analysis of TYMS antibody (10R-1166) | Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human TYMS (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: £212.33
Size: 100 ul
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