UBE2A protein (His tag) (80R-1319)
Purified recombinant Human UBE2A protein
|Synonyms||Ubiquitin conjugating enzyme E2 17 kDa protein, HHR6A protein, mHR6A protein, UBEA 2 protein, Ubiquitin protein ligase A., UBEA 2, hHR6A protein, UBE2A protein, Ubiquitin-conjugating enzyme E2 A isoform 1 BHR6A protein, UBCD6 protein, UBC2 protein, HR6A protein, RAD6A protein, UBEA-2 protein, UBEA-2, Ubiquitin conjugating enzyme E2 21.5 kDa protein, Ubiquitin conjugating enzyme E2 A protein, Ubiquitin carrier protein protein, UBE2A|
Coomassie Blue stained SDS-PAGE of UBE2A protein (His tag) (80R-1319)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-152 amino acids: MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC|
|Grade & Purity||> 95% pure|
|Molecular Weight||19.4 kDa (172aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 20% glycerol and 1mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ubiquitin-conjugating enzyme E2 A isoform 1, also known as UBE2A is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. Recombinant human UBE2A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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