UBE2K protein (His tag) (80R-1652)

Purified recombinant Human UBE2K protein

Synonyms Ubiquitin-conjugating enzyme E2 K isoform 1 protein, HIP2 protein, HYPG protein, UBEK-2 protein, UBEK 2 protein, UBEK-2, UBEK 2, UBE2K, LIG
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UBE2K protein (His tag) (80R-1652)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-200 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 26.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 1mM DTT, 10% glycerol and 50mM NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Ubiquitin-conjugating enzyme E2 K isoform 1, also known as UBE2K, is a member of the ubiquitin-conjugating enzymes family. These enzymes participate in a variety of cellular processes, including selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein UBC1. Also, UBE2K is reportedly involved in Alzheimer's disease, Huntington's disease and protein processing through its interaction with amyloid-?, huntingtin, and MHC-heavy chain proteins. Recombinant human UBE2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of UBE2K protein (His tag) (80R-1652) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £272.43
Size: 50 ug
OR
Shipping
View Our Distributors