UCHL3 protein (His tag) (80R-1631)
Purified recombinant Human UCHL3 protein
|Synonyms||Ubiquitin carboxyl-terminal hydrolase isozyme L3 protein, UCHL 3 protein, Ubiquitin thioesterase L3, UCHL3, UCHL-3 protein, UCH-L3 protein, UCHL-3, UCHL 3|
Coomassie Blue stained SDS-PAGE of UCHL3 protein (His tag) (80R-1631)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-230 amino acids: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA|
|Grade & Purity||> 95% pure|
|Molecular Weight||28.3 kDa (250aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. UCHL3 protein is 54% identical to that of UCHL1. Recombinant human UCHL3 protprot, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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