UFC1 protein (His tag) (80R-1820)

Purified recombinant Human UFC1 protein

Synonyms Ubiquitin-fold modifier-conjugating enzyme 1 protein, UFC 1 protein, UFC 1, UFC-1, UFC-1 protein, UFC1, HSPC155 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of UFC1 protein (His tag) (80R-1820)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-167 amino acids: MGSSHHHHHH SSGLVPRGSH MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 21.6 kDa (187aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1. UFM1 is activated by a novel E1-like enzyme, UBA5, by forming a high-energy thioester bond. Activated UFM1 is then transferred to its cognate E2-like enzyme, UFC1, in a similar thioester linkage. Recombinant human UFC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of UFC1 protein (His tag) (80R-1820) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £204.32
Size: 100 ug
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