Visfatin protein (His tag) (80R-1028)
Purified recombinant Mouse Visfatin protein
|Synonyms||Nicotinamide phosphoribosyltransferase [Mus musculus] protein, Pre-B-cell colony-enhancing factor 1, visfatin mouse protein, Pre-B-cell colony-enhancing factor 1 protein, Visfatin protein, mouse visfatin protein|
Coomassie Blue stained SDS-PAGE of Visfatin protein (His tag) (80R-1028)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-491 amino acids: MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN MGSSHHHHHH SSGLVPRGSH MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG WNYILEKYDG HLPIEVKAVP EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA QLNIEQDVAP H|
|Grade & Purity||> 90% pure|
|Molecular Weight||57 kDa (511 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in PBS, pH 7.4.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Visfatin is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (pre-B cell colony-enhancing factor, PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Recombinant mouse visfatin was expressed in E. coli and purified by using conventional chromatography techniques.|
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