WIBG antibody (70R-10101)
Affinity purified rabbit polyclonal WIBG antibody
|Synonyms||Polyclonal WIBG antibody, Anti-WIBG antibody, PYM antibody, MGC13064 antibody, within bgcn homolog, Drosophila antibody|
|Immunogen||WIBG antibody was raised in rabbit using the C terminal of WIBG as the immunogen|
|Assay Information||WIBG Blocking Peptide, catalog no. 33R-4474, is also available for use as a blocking control in assays to test for specificity of this WIBG antibody|
Western Blot analysis using WIBG antibody (70R-10101)
Western Blot showing WIBG antibody used at a concentration of 1-2 ug/ml to detect its target protein.
|Form & Buffer||Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.|
Usage & Assay Information
|Usage Recommendations||WB: 0.2-1 ug/ml|
Storage & Safety
|Storage||Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.|
|Biological Significance||WIBG is a key regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmarks for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. It acts as a EJC disassembly factor, allowing translation-dependent EJC removal and recycling by disrupting mature EJC from spliced mRNAs. Its association with the 40S ribosomal subunit probably prevents a translation-independent disassembly of the EJC from spliced mRNAs, by restricting its activity to mRNAs that have been translated. It interferes with NMD and enhances translation of spliced mRNAs, probably by antagonizing EJC functions.|
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