WIBG protein (His tag) (80R-1397)

Purified recombinant Human WIBG protein

Synonyms Partner of Y14 and mago, PYM protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of WIBG protein (His tag) (80R-1397)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-204 amino acids: MEAAGSPAAT ETGKYIASTQ RPDGTWRKQR RVKEGYVPQE EVPVYENKYV KFFKSKPELP PGLSPEATAP VTPSRPEGGE PGLSKTAKRN LKRKEKRRQQ QEKGEAEALS RTLDKVSLEE TAQLPSAPQG SRAAPTAASD QPDSAATTEK AKKIKNLKKK LRQVEELQQR IQAGEVSQPS KEQLEKLARR RALEEELEDL ELGLLEHHHH HH
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 23.7 kDa (212aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance WIBG has been identified as an interacting partner of Mago-Y14. The Mago-Y14 heterodimer is a core component of the EJC (exon junction complex) that is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. This protein is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. It interacts directly with Mago-Y14 by means of its N-terminal domain. Recombinant human WIBG protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of WIBG protein (His tag) (80R-1397) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: £272.43
Size: 50 ug
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